Target of this antibody:
Alternative names of antibody target:
PMAIP1 , NOXA , APR
Noxa is a BH3-only member of the Bcl-2 family. Expression of Noxa gene involves direct activation of its promotor by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Coimmunoprecipitation data sμggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as Bcl-xL and Mcl-1. Blocking the endogenous Noxa induction results in the suppression of apoptosis. These sμggest Noxa may represent a candidate mediator of p53-induced apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 100 of human Noxa
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2mg/ml) protein A purified rabbit anti-Noxa polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5% BSA, 30% glycerol, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes mainly ~16 kDa band in human, mouse and rat samples. A ~34 kDa band is also detected to the lesser extent. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.