Procathepsin K, mouse recombinant

Catalog number
1027-10
Name
Procathepsin K, mouse recombinant
Size
10 μg
Price
310.00 EUR
Supplier

Details

Target
Procathepsin K
Background
Cathepsin K is a member of the papain cysteine proteinase family and has been identified as the predominant proteinase responsible for the resorption of the bone matrix. The enzyme cleaves proteins such as collagen type I, collagen type II and osteonectin and therefore plays a role in bone remodeling and resorption in diseases such as osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.
Concentration
2.5 mg/ml
Molecular weight
35.5 kDa
Synonym
Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
Other name
Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
NCBI gene number
1513
NCBI gene
CATK
Gene source
Mouse
NCBI number
P43235
Recombinant
Yes
Source
E. coli.
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
>1000 mU/mg (1 U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100).
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
1 U = 1 µmole/min/mg
Storage condition
-80°C
Shipping under
Dry ice
Physical appearance
Liquid
Physical properties
2.5 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants