Procathepsin K, human recombinant

Catalog number
1026-10
Name
Procathepsin K, human recombinant
Size
10 μg
Price
310.00 EUR
Supplier

Details

Target
Procathepsin K
Background
Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.
Concentration
1 mg/ml
Molecular weight
35.9 kDa
Synonym
CTSK, CTSO, CTSO3
Other name
CTSK, CTSO, CTSO3
NCBI gene number
1513
NCBI gene
CATK
Gene source
Human
NCBI number
P43235
Recombinant
Yes
Source
E. coli.
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
The specific activity is>1000 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)
Reesults
The specific activity is>1000 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)
Binding ability
N/A
Unit
1U = 1 µmole/min/mg
Storage condition
-80°C
Shipping under
Dry ice
Physical appearance
Liquid
Physical properties
1 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants