Target of this antibody:
Alternative names of antibody target:
IL17A/F, IL17 A/F, IL-17A/F, IL-17 A/F, IL17AF, IL-17, AF, Interleukin-17 A/F, Interleukin-17 AF
IL-17F, a member of the IL-17 family of structurally related cytokines, has been shown to stimulate proliferation and activation of T-cells and PBMCs. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
Clonality of antibody:
This antibody reacts with:
E.coli derived Recombinant Human IL-17F
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
A sterile filtered antibody solution in PBS, pH 7.2.
This antibody has been succesfully tested for use in following methods:
Western blot, ELISA, Neutralization
1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-17F is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution) 3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17F (50.0 ng/ml), a concentration of 0.9-1.2 μg/ml of this antibody is required.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.