3-Nitrotyrosine ELISA Kit

Catalog number
K4158-100
Name
3-Nitrotyrosine ELISA Kit
Size
100 assays
Price
683.00 EUR
Supplier

Details

Kit's description
3-Nitrotyrosine (3-NT) is a biomarker of nitrogen free radical species modified proteins in systemic autoimmunogenic conditions. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: Universal • Application: quantitative measurement of 3:Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Kit's benefits
Easy, convenient and time-saving method to measure the level of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Category
ELISA kit
Kit's other name
3-Nitrotyrosine ELISA Kit
Contents
• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers
Storage condition
4°C
Shipping condition
gel pack
Maximum time can be stored
6 months
Species reactivity
Universal
Applications
Quantitative measurement of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Samples tested
Serum, plasma, tissue homogenates, cell culture supernatant and other biological fluids.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED