Human CellExp™ ErbB3/HER3, human recombinant

Catalog number
7400-10
Name
Human CellExp™ ErbB3/HER3, human recombinant
Size
10 μg
Price
246.00 EUR
Supplier

Details

Target
ErbB3
Background
ErbB3, also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
Concentration
N/A
Molecular weight
This protein is fused with a C-terminal 6×his tag and has a calculated MW of 71.5 kDa after removal of signal peptide. The predicted N-terminal is Ser 20. Protein migrates as 100-110 kDa in reduced SDS-PAGE resulting from glycosylation.
Synonym
ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3
Other name
ERBB3, ErbB-3, HER3, HER-3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, Receptor tyrosine-protein kinase erbB-3
NCBI gene number
2065
NCBI gene
ErbB3
Gene source
Human
NCBI number
P21860
Recombinant
Yes
Source
HEK293 cells
Purification
≥95%
Tested applications
SDS-PAGE, N/A
Tested activity
N/A
Biological activity
Measured by its ability to inhibit the biological activity of Neuregulin1-β1 on MCF-7 human breast cancer cells, in the presence of 10 ng/ml recombinant Human NRG1-β1/HRG1-β1 Extracellular Domain. The ED50 for this effect is typically 0.5-3.0 μg/ml
Reesults
Measured by its ability to inhibit the biological activity of Neuregulin1-β1 on MCF-7 human breast cancer cells, in the presence of 10 ng/ml recombinant Human NRG1-β1/HRG1-β1 Extracellular Domain. The ED50 for this effect is typically 0.5-3.0 μg/ml
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants