Biotin-DEVD-FMK for cell activation

Catalog number
1124-20C
Name
Biotin-DEVD-FMK for cell activation
Size
20 µl (10 mM)
Price
371.00 EUR
Supplier

Details

Description
A synthetic peptide that irreversibly inhibits activity of caspase-3 and related caspases and blocks apoptosis. The inhibitor is designed as a methyl ester to enhance cell permeability. The conjugation with biotin allows detection of activated caspases in conjunction with avidin-tags. (CAUTION: If the intended use is on purified or recombinant enzymes, esterase should be added to generate free carboxyl groups).
Peptide sequence
Biotin-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK
CAS number
N/A
Molecular weight
873.99
Category
Caspase Inhibitor
Other name
Biotin-DEVD-FMK, DEVD-FMK, Biotin-
Molecular formula
C₃₈H₆₀FN₇O₁₃S
Physical appearance
Liquid
Supplied with
DMSO (10 mM)
Is this a salt?
No
Is it cell-permeable?
Yes
Purification
>95% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Storage instructions
Protect from light and moisture
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
For cells, cell lines and tissues in culture till half confluency.