Target of this antibody:
Alternative names of antibody target:
BIN1 , AMPH2 , amphiphysin-like , MGC10367 , DKFZp547F068 , AMPHL , SH3P9
BIN-1 is a tumor suppressor protein that was identified in a genetic screen for proteins that interact with the MCY oncoprotein. BIN-1 inhibits the oncogenic activity of MYC, possibly throμgh activating or facilitating apoptosis. BIN is a short-lived protein with a half-life of about 2 hours.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide corresponding to residues surrounding amino acids 578 of mouse BIN-1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2mg/ml) immunoaffinity purified rabbit anti-Bin-1 polyclonal antibody in phosphate-buffered saline (PBS) containing 0.1% BSA, 30% glycerol, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~65 kDa band in samples from human, mouse and rat origins. A cleavage fragment (~45 kDa) can also be detected in apoptotic samples. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.