Target of this antibody:
Alternative names of antibody target:
RAC serine/threonine-protein kinase; DRAC-PK; Dakt1; DAkt; Protein kinase B; PKB
Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence sμggests that Akt functions to promote cell survival by actively inhibiting apoptosis, althoμgh the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Phopho-Akt (Ser473) polyclonal antibody in phosphate buffered saline (PBS), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.