Biotinylated AGE-BSA

Catalog number
7929-250
Name
Biotinylated AGE-BSA
Size
250 µg
Price
208.00 EUR
Supplier

Details

Highlights
FORM: Liquid. FORMULATION: 5 mg/ml of 0.22 µm filter sterilized solution in 1X PBS LABELING EFFICIENCY: BioVision’s Biotinylated AGE-BSA was estimated to contain ~1-2 biotin/BSA (As tested using Biotin Quantitation Kit (K811-100)). APPLICATIONS: Biotinylation of AGE-BSA allows for its pull-down and purification by Streptavidin-Sepharose beads (Cat. # 6565).
Description
Advanced glycation end products (AGEs) accumulate with age and at an accelerated rate in diabetes. AGEs bind cell-surface receptors including the receptor for AGE (RAGE). AGEs therefore have been implicated in the pathogenesis of diabetes, induction of proinflammatory cytokines, and stimulation of smooth muscle proliferation, and fibronectin production. The Biotinylated AGE-BSA was produced by biotinylation of AGE-BSA (Cat. # 2221-10) with Sulfo-NHS-LC-Biotin (Cat. # 2326). Prior to biotinylation, AGE-BSA was produced by reacting BSA with Glycoaldehyde under sterile conditions followed by extensive dialysis and purification steps. Fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex./Em. = 370/440 nm. Glycated BSA shows a 7000% increase in fluorescence in compared to control BSA.
Category
AGE-BSA
Storage condition
-70°C
Shipping condition
gel pack
Maximum time for storage
12 months