anti-sRANK Ligand Antibody

Catalog number
5111-50
Name
anti-sRANK Ligand Antibody
Size
sRANK Ligand Antibody
Price
311.00 EUR
Supplier

Details

Target of this antibody:
sRANKL
Alternative names of antibody target:
soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)
Background information:
RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Mouse
Immunogene sequence:
E.coli derived Recombinant Murine sRANKL
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
A sterile filtered antibody solution in PBS, pH 7.2.
Purification method:
Affinity chromatography
This antibody has been succesfully tested for use in following methods:
Western blot, ELISA, Neutralization
Recommended usage:
1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant murine sRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution) 3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of msRANKL (50.0 ng/ml), a concentration of 0.05 μg/ml of this antibody is required.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps