Target of this antibody:
Alternative names of antibody target:
CDC42Hs , G25K
cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also sμggest that constitutive active forms of cdc42 can induce apoptosis throμgh a mechanism requiring signaling throμgh SAPK/JNK.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, bovine
Synthetic peptides surrounding amino acid 144 of human cdc42
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-cdc42 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.