TNF-alpha, human recombinant

Catalog number
1050-1000
Name
TNF-alpha, human recombinant
Size
1 mg
Price
1670.00 EUR
Supplier

Details

Target
TNF-alpha
Background
Tumor Necrosis Factor-α(TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-α is a 17.4 kDa protein containing 157 amino acid residues.
Concentration
N/A
Molecular weight
17.4 kDa
Synonym
TNF, Tumor necrosis factor- alpha
Other name
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
NCBI gene number
7124
NCBI gene
TNFA
Gene source
Human
NCBI number
P01375
Recombinant
Yes
Source
E. coli.
Purification
≥98%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <0.05 ng/ml, corresponding to a specific activity of> 2 x 10⁷ units/mg.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
gel pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized from a solution of 3 mM Tris, pH 8.0
How to reconstitute
Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
The TNF-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.