FGF-2/FGF-basic, rat recombinant

Catalog number
4039-10
Name
FGF-2/FGF-basic, rat recombinant
Size
10 μg
Price
137.00 EUR
Supplier

Details

Target
FGF-2
Background
FGF-2 is a basic heparin binding growth factor that stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. The recombinant rat FGF-2 is a single, non-glycosylated chain containing 145 amino acids and having a molecular weight of 16.3 kDa.
Concentration
N/A
Molecular weight
16.3 kDa
Synonym
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast Growth Factor
Other name
FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
NCBI gene number
54250
NCBI gene
FGF-2
Gene source
Rat
NCBI number
P13109
Recombinant
Yes
Source
E. coli
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The ED₅₀, calculated by the dose-dependent proliferation of BALB/3T3 cells is
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Lyophilized from 1 mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130 mM NaCl.
How to reconstitute
Reconstitute in sterile H₂O to 0.1–1 mg /ml. The solution can then further diluted to other aqueous solutions
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Additional source
Recombinants or rec. proteins
Group
recombinants