Annexin V-FITC Reagent

Catalog number
1001-200
Name
Annexin V-FITC Reagent
Size
200 assays
Price
403.00 EUR
Supplier

Details

Highlights
•Detection method- flow cytometry (Ex/ Em= 488 /530 nm) using FL1 channel and fluorescence microscopy •Sample- Cell culture •Formulation- 0.15 mg/ml in PBS containing 1% BSA, 0.02% sodium azide •Simple, easy to use and convenient
Description
Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry.
Category
Annexin V Apoptosis Assay
Storage condition
+4°C
Shipping condition
gel pack
Maximum time for storage
12 months
Additional description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.
Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
Conjugation
Anti-FITC Antibody