Target of this antibody:
Alternative names of antibody target:
SMC2, structural maintenance of chromosomes 2; SMC2L1, structural maintenance of chromosomes 2-like 1; CAPE, CAP-E, hCAP-E, human chromosome-associated protein E
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core condensin complex includes a heterodimer of SMC2 and SMC4 that functions to create positively supercoiled loops for chromatin condensation and metaphase chromatid shaping. A function for condensin in the DNA damage checkpoint response and gene repression has also been suggested.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human SMC2
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1:500 – 1:2000). However, the optimal concentrations should be determined individually. HEK293T cell lysate can be used as a positive control.The antibody recognizes the SMC2 of human origin. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.