Ready-to-Use Ni QR Agarose Beads Buffer Kit

Catalog number
K6563-3
Name
Ready-to-Use Ni QR Agarose Beads Buffer Kit
Size
3 components
Price
153.00 EUR
Supplier

Details

Highlights
• Contents: Ni QR Agarose Loading Buffer, Elution Buffer & Extraction Reagent • Sufficient for purification of up to 300 mg of target protein using 5 ml of Hi-Bind™ Ni QR Agarose Beads (Cat. # K6562) or equivalent under batch/gravity flow conditions. • Applications: - Purification of native and recombinant proteins and peptides that have an affinity for metal ions - For purifications under native conditions
Description
Basic research of the physico-chemical properties as well as the activity of proteins is impossible without their isolation and purification. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during purification as well as the low cost of purification made this type of fusion the most popular (and in many cases, the first tag of choice). BioVision’s Ready-to-Use Ni QR Agarose Beads Buffer Kit works seamlessly with Hi-Bind™ Ni QR Agarose Beads (Cat. # 6562) or equivalent from the isolation of target to obtaining highly purified and biologically active protein. These Buffers & Extraction Reagent deliver efficient extraction of target protein (as high as 96% efficiency of SDS boiling method) while preserving its integrity. The buffer components are designed to minimize the metal ion leaching while preserving the biological activity of precious target proteins. This kit is sufficient for purification of up to 300 mg of target protein using 5 ml of Hi-Bind™ Ni QR Agarose Beads (Cat. # K6562) or equivalent under batch/gravity flow conditions.
Category
ni qr agarose beads buffer kit
Storage condition
+4°C
Shipping condition
gel pack
Maximum time for storage
12 months
Properties
The purest agarose was used in the production of - - Ni QR Agarose Kit by Biovision.
Conjugation
Agarose
Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Biovision.