HeLa Nuclear Extract

Catalog number
1641-1
Name
HeLa Nuclear Extract
Size
1 mg
Price
327.00 EUR
Supplier

Details

Highlights
• Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF. •Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay
Description
Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
Category
HeLa Nuclear Extract
Storage condition
-70°C
Shipping condition
dry ice
Maximum time for storage
12 months
Additional description
Pure extracts used in broths or as control for cell culture and Western Blot.