Target of this antibody:
Alternative names of antibody target:
SBP-Tag, anti-SBP-Tag, SBP-Tag antibody,SBP-Tag antibody
Streptavidin Binding Peptide (SBP) tag is very versatile and can be inserted into expression vectors for easy detection of recombinant proteins and purification by use of affinity columns. The full sequence for SBP-tag is 38 amino acids long and exhibits a nanomolar scale binding affinity for streptavidin.
Clonality of antibody:
This antibody reacts with:
A synthetic SBP peptide CEQLRARLEHHPQGQREP conjμgated to KLH.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-SBP-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.