Target of this antibody:
Alternative names of antibody target:
PPARA , PPAR , OTTHUMP00000042872 , hPPAR , MGC2452 , OTTHUMP00000028713 , NR1C1 , PPAR-alpha , MGC2237
PPAR (Peroxisome proliferator-activated receptor) is a member of the nuclear hormone receptor superfamily and functions as a transcriptional activator. PPARα is preferentially expressed in liver, skeletal muscle, heart and kidney. PPARα is involved in regulation of lipid homeostasis. Its ligands include fatty acids, NSAIDs, prostaglandins, leukotriene B4, etc. PPARα transcriptionally regulates a variety of genes for enzymes and proteins involved in fatty acid metabolism and oxidation.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, bovine, porcine
Synthetic peptide surrounding amino acid 25 of human PPARα
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% ProClin®.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~52 kDa PPARα in human sample. A ~28 kDa cleavage fragment can also be detected in samples of human, mouse, and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
The anti-PPAR-alpha Antibody is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.