Target of this antibody:
Alternative names of antibody target:
Alpha-1, 2-mannosidase EDEM3
EDEM3 belongs to the glycosyl hydrolase 47 family. It is involved in endoplasmic reticulum-associated degradation (ERAD). Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 accelerates the degradation of misfolded glycoproteins in the ER. It also accelerates the glycoprotein ERAD by proteasomes and this process depends on mannose-trimming from the N-glycans. It is most likely to have some alpha 1, 2-mannosidase activity.
Clonality of antibody:
This antibody reacts with:
Mouse, rat, human
Synthetic peptide at C-terminal (BV-M5)
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH7.2
This antibody has been succesfully tested for use in following methods:
Western blot: 1:200
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.