BRD2 bromodomain 1 and 2 (65-459 aa) (GST-tagged), human recombinant

Catalog number
7647-100
Name
BRD2 bromodomain 1 and 2 (65-459 aa) (GST-tagged), human recombinant
Size
100 μg
Price
795.00 EUR
Supplier

Details

Target
BRD2
Background
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Concentration
N/A
Molecular weight
71.2 kDa (65-459 aa + GST Tag)
Synonym
Human recombinant BRD2 bromodomain 1 and 2 (65-459 aa) (GST-tagged)
Other name
RING3; RNF3; Bromodomain containing 2
NCBI gene number
6046
NCBI gene
BRD2
Gene source
Human
NCBI number
P25440
Recombinant
Yes
Source
E. coli
Purification
≥90%
Tested applications
SDS-PAGE, N/A
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-80°C
Shipping under
dry ice
Physical appearance
Liquid
Physical properties
50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants