Target of this antibody:
Alternative names of antibody target:
CARM1, CARM-1, Coactivator-Associated Arginine Methyltransferase 1, PRMT4, Protein Arginine N-methyltransferase 4
CARM1 (coactivator-associated arginine methyltransferase 1), also known as protein arginine N-methyltransferase 4 (PRMT4), is a 585 amino acid nuclear and cytoplasmic protein belonging to the protein arginine N-methyltransferase family. As a protein arginine N-methyltransferase, CARM1 is capable of catalyzing the transfer of methyl groups from S-adenosylmethionine to the guanidino group nitrogen atoms of arginine residues in certain proteins involved in mRNA stability, DNA packaging and transcriptional regulation. The methyltransferase activity of CARM1 has been found to be negatively regulated through phosphorylation at a conserved serine residue. CARM1 acts as a positive regulator for multiple transcription factors and functions as a secondary coactivator through its association with p160 coactivators. CARM1 exists as two alternatively spliced isoforms, and is encoded by a gene that maps to human chromosome 19p13.2.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 569 of human CARM1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~63kDa CARM1on samples of human, mouse and rat origin. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.