Mouse Monoclonal Anti-RAD9A Antibody (3A3-A7-F8)

Catalog number
A1316-100
Name
Mouse Monoclonal Anti-RAD9A Antibody (3A3-A7-F8)
Size
100 µg
Price
338.00 EUR
Supplier

Details

Description
Mouse Monoclonal antibody to detect RAD9A
Raised in
Mouse
Clonality
Monoclonal Antibody (Clone#3A3-A7-F8)
Reacts with
Human
Immunogen
Recombinant human RAD9A protein fragments expressed in E.coli
Target
RAD9A (hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A, Cell cycle checkpoint control protein RAD9A)
Research area
Epigenetics
Gene number
5883
Protein number
Q99638
Physical state
Colorless liquid
Package form
Affinity purified antibody is supplied in liquid form in PBS (pH 7.4) containing with 0.2% sodium azide, 50% glycerol
Purity
Affinity purified
Antibody's positive controles
Western blot: HeLa, MCF7, 3T3, COS7 and Rat testis cell lysates; Immunoprecipitation: HeLa and 3T3 cells
Approved applications with antibody dilution
Western blot; 1:500, Immunoprecipitation
Scientific context
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Delivery
Shipped to your lab under gel pack
Precautions
Manifactured in USA. For Research Use Only! Not to be used in humans
Released
This antibody is available to order since May 2017
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
French translation
anticorps