Caspase-3, rat recombinant

Catalog number
1283-25
Name
Caspase-3, rat recombinant
Size
25 units
Price
208.00 EUR
Supplier

Details

Target
Caspase-3
Background
Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).
Concentration
N/A
Molecular weight
large (17 kD) and small (11 kD) subunits
Synonym
Caspase 3
Other name
Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, IRP, LICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1
Gene source
Rat
Recombinant
Yes
Source
E. coli
Purification
N/A
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
300,000 units/mg
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Storage condition
-70°C
Shipping under
dry ice
Physical appearance
Lyophilized powder
Physical properties
Lyophilized powder
How to reconstitute
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Additional source
Recombinants or rec. proteins
Group
recombinants