Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)

Catalog number
7098-25
Name
Biotinylated Bovine Serum Albumin (Biotin-LC-BSA) (5 biotin/BSA)
Size
25 mg
Price
234.00 EUR
Supplier

Details

Highlights
Biotinylated BSA is used as a control in biotin estimation procedures and as a competitive inhibitor for anti-biotin antibodies. It has 5 moles of biotin/mole of BSA. It is provided as a 2 mg/ml solution in 50 mM Na2HPO4, 100 mM NaCl pH 7.5.
Description
Biotinylated BSA is synthesized through biotinylation of BSA with Sulfo-NHS-LC-Biotin (Cat # 2326). It has 5 moles biotin/mole of BSA as determined by Biovision’s Biotin Estimation Kit, Catalog # K811-50, 100. It can be used as a positive control in biotin estimation procedures and as a competitive inhibitor for anti-biotin antibodies.
Category
BSA
Storage condition
+4°C
Shipping condition
gel pack
Maximum time for storage
12 months
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
Group
Sera
About
Serum should be stored at +5°C