Target of this antibody:
Alternative names of antibody target:
Prohibitin is an evolutionarily conserved protein that has anti-proliferative activity. Prohibitin is a 30 kDa, post-synthetically modified protein found in the inner membrane of mitochondria and on the plasma membrane of B cells. It mediates B cell mutation. Prohibitin Controls cell cycle in both transcriptional and post-translational mechanisms. Prohibitin is a potential tumor suppressor. Prohibitin gene mutations are correlated with the breast cancer development and/or progression in over 80%0f the cell lines analyzed.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 256 of human Prohibitin
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunofluorescence
Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunofluorescence (5-10 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 33 kDa protein, corresponding to the apparent molecular mass of Prohibitin on SDS-PAGE immunoblots, in samples from human, mouse and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.