anti-GM-CSF Antibody

Catalog number
5102-50
Name
anti-GM-CSF Antibody
Size
GM-CSF Antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
GM-CSF
Alternative names of antibody target:
CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, MGC131935
Background information:
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Mouse
Immunogene sequence:
E.coli derived Recombinant Murine GM-CSF
Concentration of antibody:
N/A
Form of antibody:
Liquid
Formulation of this antibody:
A sterile filtered antibody solution in PBS, pH 7.2.
Purification method:
Affinity chromatography
This antibody has been succesfully tested for use in following methods:
Western blot, ELISA, Neutralization
Recommended usage:
1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution) 3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 µg/ml of this antibody is required
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Gene
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.
French translation
anticorps