Target of this antibody:
Alternative names of antibody target:
RAF1 , cRaf , Raf-1 , c-Raf , RAF , CRAF , C-RAF, RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
c-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-Raf (Ser259) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (0.5-4 µg/ml) and Immunohistochemistry (20-40µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.