anti-Caspase-7 Antibody

Catalog number
3135-100
Name
anti-Caspase-7 Antibody
Size
Caspase-7 Antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
Caspase-7
Alternative names of antibody target:
CASP7,CASP-7,ICE-LAP3,CMH-1,MCH3, EC 3.4.22.60
Background information:
Caspase-7 is a 34 kDa protein consisting of two subunits and a short prodomain. The procaspase-7 can be activated by cleavage by upstream caspases to form active caspase-7 in apoptosis. Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase (PARP) and sterol regulatory element binding proteins (SREBPs).
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7 large fragment
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (200 µg/ml) affinity purified rabbit anti-caspase-7 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the full length (35-37 kDa) and the large fragment (20-25 kDa) of processed caspase-7 by Western blotting. Mouse small intestine tissue lysate can be used as a positive control.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps