PCAF bromodomain (492-658 aa), human recombinant

Catalog number
1137-500
Name
PCAF bromodomain (492-658 aa), human recombinant
Size
500 μg
Price
1023.00 EUR
Supplier

Details

Target
HAT/PCAF
Background
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. P300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
Concentration
N/A
Molecular weight
N/A
Synonym
p300/CBP-associated factor; KAT2B
Other name
p300/CBP-associated factor; KAT2B
NCBI gene number
8850
NCBI gene
PCAF
Gene source
Human
NCBI number
Q92831
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)
Biological activity
≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)
Reesults
≥0.2 U/mg
Binding ability
N/A
Unit
One unit of HAT activity is the amount of enzyme that generates 1.0 µmol of CoA per minute at 30°C under kit assay conditions.
Storage condition
-20°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
30% Glycerol in PBS
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants