Caspase-1, mouse recombinant

Catalog number
1181-25
Name
Caspase-1, mouse recombinant
Size
25 units
Price
240.00 EUR
Supplier

Details

Target
Caspase-1
Background
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).
Concentration
N/A
Molecular weight
large (20 kD) and small (10 kD) subunits.
Synonym
Caspase 1
Other name
Caspase-1, Short name=CASP-1, Interleukin-1 beta convertase, Short name=IL-1BC, Interleukin-1 beta-converting enzyme, Short name=ICE, IL-1 beta-converting enzyme, p45
NCBI gene number
12362
NCBI gene
CASP1
Gene source
Mouse
NCBI number
P29452
Recombinant
Yes
Source
E. coli
Purification
N/A
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
20000 units/mg
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Storage condition
-70°C
Shipping under
gel pack
Physical appearance
Semi-Dry
Physical properties
Semi-Dry
How to reconstitute
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
Additional source
Recombinants or rec. proteins
Group
recombinants