Target of this antibody:
Alternative names of antibody target:
Cyclooxygenase 1, PTGS1, PCOX1, COX-1, PGG/HS, PGHS1 , PGHS-1, PHS1 , COX1, PTGHS
COX proteins are membrane-associated heme proteins that have cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Nonsteroidal anti-inflammatory drμgs) such as aspirin. Prostaglandins (PGs) formed by the enzymatic activity of COX-1 are primarily involved in the regulation of homeostatic functions throμghout the body, whereas PGs formed by COX-2 primarily mediate pain, fever, and inflammation. COX-1 is constitutively expressed, with particularly high expression in gastrointestinal tissues. COX-2 is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide corresponding to residues surrounding amino acids 586 of mouse Cox-1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (1-4 µg/ml), immunoprecipitation, and Immunohistochemistry(5µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 70 kDa COX-1 of human, mouse and rat origins. It does not cross-react with COX-2.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.