anti-HA-Biotin Antibody (Clone 2C16)

Catalog number
3996B-100
Name
anti-HA-Biotin Antibody (Clone 2C16)
Size
HA-Biotin Antibody (Clone 2C16)
Price
360.00 EUR
Supplier

Details

Target of this antibody:
HA-Tag-Biotin
Alternative names of antibody target:
GRIN2A , hNR2A , NMDAR2A , NR2A
Background information:
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG
Clone:
2C16
This antibody reacts with:
All species
Immunogene sequence:
Synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified anti-HA-Biotin monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunofluorescence
Recommended usage:
Western blot analysis, immunoprecipitation, and localization of recombinant HA-tagged proteins by standard immuno detection methods (2-10 µg/ml) using Avidin-FITC or other tags. However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
French translation
anticorps