FGF-2/FGF-basic, bovine recombinant

Catalog number
4040-10
Name
FGF-2/FGF-basic, bovine recombinant
Size
10 μg
Price
148.00 EUR
Supplier

Details

Target
FGF-2
Background
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
Concentration
N/A
Molecular weight
17.25 kDa
Synonym
FGF-2, FGF2
Other name
FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
NCBI gene number
281161
NCBI gene
FGF-2
Gene source
Bovine
NCBI number
P03969
Recombinant
Yes
Source
E. coli
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Sterile filtered white lyophilized powder. Prepared fom a sterile solution containing 1 % HSA.
How to reconstitute
Reconstitute in sterile water to a concentration not less than 0.1 mg/ml. Aliquot and store at –20°C. Avoid freeze-thaw cycles.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
Additional source
Recombinants or rec. proteins
Group
recombinants