Active Sonic Hedgehog (SHH), murine recombinant

Catalog number
4020-100
Name
Active Sonic Hedgehog (SHH), murine recombinant
Size
100 μg
Price
697.00 EUR
Supplier

Details

Target
Sonic Hedgehog (SHH)
Background
Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.
Concentration
N/A
Molecular weight
19.8 kDa
Synonym
SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Other name
SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
NCBI gene number
20423
NCBI gene
SHH
Gene source
Mouse
NCBI number
Q8C765
Recombinant
Yes
Source
E. coli
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
Mouse SHH is fully biologically active when compared to standard. Determined by it’s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED₅₀ in the range of 0.03 – 0.30 ug/ml.
Reesults
0.03 – 0.30 ug/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
How to reconstitute
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Additional source
Recombinants or rec. proteins
Group
recombinants