anti-Phospho-FoxO4/AFX Antibody

Catalog number
3270-100
Name
anti-Phospho-FoxO4/AFX Antibody
Size
Phospho-FoxO4/AFX Antibody
Price
403.00 EUR
Supplier

Details

Target of this antibody:
Phospho-FoxO4/AFX
Alternative names of antibody target:
AFX1, MLLT7, Forkhead box protein O4; Fork head domain transcription factor AFX1
Background information:
The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX, AND FKHRL1) have sequence similarity to the nematode homolog Daf 16, recently shown to mediate signaling via a pathway involving IGFR1, P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site, FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse
Immunogene sequence:
Synthetic peptide corresponding to residues surrounding Ser193 of human FoxO4/AFX
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified polyclonal rabbit anti-Phospho-AFX antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blotting (1-2 µg/ml). However, the optimal concentrations should be determined individually. Phospho-AFX (Ser193) antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps