Target of this antibody:
Alternative names of antibody target:
MCP1, MCP-1, MCP 1, monocyte chemoattracctant protein-1, monocyte chemoattracctant protein 1, monocyte chemoattracctant protein
Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a N-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con-A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than human MCP-1 at 3’-end. The 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and which explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophiles. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
Clonality of antibody:
This antibody reacts with:
E. coli-expressed rat MCP-1 (a.a. 1-73)
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-rat MCP-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, Neutralization
Western blot analysis (0.5-2 µg/ml). From researcher’s feedback, it can also be used for neutralization studies. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.