anti-Caspase-7 Antibody (Clone B4-G2)

Catalog number
3010-100
Name
anti-Caspase-7 Antibody (Clone B4-G2)
Size
Caspase-7 Antibody (Clone B4-G2)
Price
371.00 EUR
Supplier

Details

Target of this antibody:
Caspase-7
Alternative names of antibody target:
CASP7,CASP-7,ICE-LAP3,CMH-1,MCH3, EC 3.4.22.60
Background information:
The caspase family of cysteine proteases plays a key role in apoptosis. Caspase-7, also known as Mch3α, ICE/LAP3 and CMH1, is a 34 kDa protein that is activated during Fas- and TNF-induced apoptosis. Caspase-7 has been identified as a major contributor to the execution phase of cellular apoptosis. It cleaves essential cellular machinery following its own cleavage from an inactive proenzyme to an active heterodimeric form. Caspase-7 is processed by caspase-3, -6, -8, -9, and –10 as well as by Granzyme B.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1
Clone:
B4-G2
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide from human caspase-7.
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Recommended usage:
Western blot analysis (0.5-2 µg/ml) and immunoprecipitation (4-10 µg/ml). However the optimal conditions should be determined individually. Detects both pro-caspase-7 (34 kDa) and the large subunit of cleaved caspase-7 (19 kDa) in apoptosis cells on SDS-PAGE immunoblots.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps