anti-Caspase-9 (Active) Antibody

Catalog number
3149-100
Name
anti-Caspase-9 (Active) Antibody
Size
Caspase-9 (Active) Antibody
Price
469.00 EUR
Supplier

Details

Target of this antibody:
Caspase-9 (Active)
Alternative names of antibody target:
CASP-9, EC 3.4.22.62, ICE-like apoptotic protease 6,ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3, Apaf-3
Background information:
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human
Immunogene sequence:
Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 0.5% BSA, and 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Recommended usage:
Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal concentrations should be determined individually. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps