Caspase-9 Proform, human recombinant

Catalog number
1089P-50
Name
Caspase-9 Proform, human recombinant
Size
50 μg
Price
447.00 EUR
Supplier

Details

Target
Caspase-9
Background
Caspase-9 is a member of the caspase family of cysteine proteases. Caspase-9 exists in cells as an inactive proenzyme. Proteolytic processing of this inactive proenzyme generates the large and small subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be the key signal-transducing events in apoptosis. Caspase-9 is considered to be an upstream enzyme that initiates caspase cascade leading to apoptosis. The recombinant Procaspase-9 is produced by expression of a human cDNA in E. coli and further purified by multiple chromatographic steps.
Concentration
2μg/μl
Molecular weight
N/A
Synonym
Caspase-9 Proform
Other name
Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, LEHDase
Gene source
Human
Recombinant
Yes
Source
E. coli.
Purification
≥90%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-70°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
2 µg/µl in 1 mM acidic acid
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants