JNK Activated Cell Lysate

Catalog number
7032-1
Name
JNK Activated Cell Lysate
Size
1 mg
Price
295.00 EUR
Supplier

Details

Highlights
• CONCENTRATION- 4 mg/ml
Description
Jurkat cells are cultured in RPMI medium supplement with 10% FBS. The cells were grown to late growing phase. Anisomycin is added to the medium to a final concentration 10 µg/ml (BioVision cat# 1549-10, dissolved in DMSO to 10 mg/ml as stock). The cells are incubated at 37°C for 15 minutes, then collected and lysed in Extraction Buffer (BioVision Cat # K430-40). The cell lysate can be used as a positive control for JNK activity assays in conjunction with BioVision’s JNK Activity Assay Kit (K430-40 or K431-40). For positive controls, we recommend using 100 µg per assay.
Category
JNK
Storage condition
-70°C
Shipping condition
dry ice
Maximum time for storage
6 months
Additional description
For cells, cell lines and tissues in culture till half confluency.