Target of this antibody:
Alternative names of antibody target:
Bax-interacting factor 1 , Bif-1 , CGI-61 , Endophilin 3 , Endophilin B1 , KIAA0491 , SH3GLB1 , SH3p13
BIF-1 (Bax interacting factor-1) is a 365 amino acid protein that interacts with Bax protein and influences cell survival and death. BIF-1 contains a Src homology 3 (SH3) domain in the C-terminus. Overexpression of BIF-1 promotes Bax conformational change, caspase activation, and apoptotic cell death.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 351 of human BIF
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-BIF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 40 kDa BIF-1 in samples from human, mouse, and rat origins. Jurkat cell lysate and rat kidney tissue lysates can be used as positive controls.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.