Target of this antibody:
Alternative names of antibody target:
Blue Fluorescent Protein
The Blue Fluorescent Protein (BFP) can be used as protein marker to study subcellular localization or translocation of proteins.
Clonality of antibody:
This antibody reacts with:
Recombinant BFP expressed from E. coli
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunofluorescence
Western blot analysis (0.5-4 µg/ml). Recombinant BFP (Cat.#: 4994-10) can be used as a positive control. Based on researchers' feedback, it can also be used in immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.