Target of this antibody:
Alternative names of antibody target:
TBC1D4; AS160; DKFZp779C0666
AS160 is a 160 kDa Akt serine/threonine kinase substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. Increased in insulin treatment stimulate phophorylation of AS160 that regulates GLUT4 translocation via inactivation of the Rab GTPase function.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 186 of human AS160/TBC1.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-AS160/TBC1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blotting (1–4 µg/ml).
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.