Protein G Sepharose Column

Catalog number
6518-1
Name
Protein G Sepharose Column
Size
1 ml
Price
262.00 EUR
Supplier

Details

Highlights
•CONTENTS- Ready-to-use pre-packed columns of 1 ml or 5 ml bead volume in PBS with 0.02% sodium azide. • FEATURES: - HIGH BINDING CAPACITY: Binding of IgG>20 mg human or rabbit IgG/ml Protein G-Sepharose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 2.07 ml/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Description
Protein G-Sepharose Columns are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose beads. BioVision Protein G (Cat. # 6510) is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G-Sepharose Column is>20 mg of human or rabbit IgG per ml of wet beads. Protein G-Sepharose Columns display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.
Category
Protein G
Storage condition
+4°C
Shipping condition
gel pack
Maximum time for storage
12 months