Target of this antibody:
Alternative names of antibody target:
Uncoupling protein 1, mitochondrial brown fat uncoupling protein 1, solute carrier family 25 member 7, SLC25A7, Thermogenin
The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
UCP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human UCP1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
1 mg/ml in PBS containing 0.02% sodium azide
Affinity chromatography purified via peptide column
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, ELISA
Western blot: 1 - 2 µg/mL, IHC: 2.5 µg/mL, ELISA
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.