Target of this antibody:
Alternative names of antibody target:
Breast Cancer Susceptibility Protein 2, Early Onset; FANCB, FANCD1, FACD , FANCB , FAD1 , FAD , FANCD1 , BRCC2 , OTTHUMP00000042401
BRCA 1 and 2 are breast cancer susceptibility proteins that play a role in DNA damage, repair, cell cycle progression, transcription and apoptosis, etc. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. BRCA2 has been shown to be required for localization of Rad51 to sites of DSBs (double stranded breaks) in DNA. Cells lacking BRCA1 and BRCA2 cannot repair DSBs throμgh the Rad51-dependent process.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide mapping to the N-terminus of human BRCA2
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-BRCA2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.