Target of this antibody:
Alternative names of antibody target:
RNPC2, RNA-binding region containing protein 2, Caper, Coactivator of activating protein-1 and estrogen receptors, HCC1, Hepatocellular carcinoma protein 1, Splicing factor CC1.3, Splicing factor CC1.4
Caper, also known as splicing factor HCC1 or hepatocellular carcinoma protein 1 and RNA binding region containing protein 2 (RNPC2), acts as a transcriptional coactivator for steroid nuclear receptors c-Jun, ERalpha and ER-beta. Caper, a nuclear protein with highest concentrations in nuclear speckles, plays a role in the pre-mRNA splicing process. Two isoforms of Caper, HCC1.3 and HCC1.4, co-localize with pre-mRNA splicing factor SC35 and uridine-rich small nuclear RNAs. Caper is a widely expressed protein with highest levels detected in skeletal muscle, lung, brain and pancreas.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide mapping to range between 325-375 of human Caper
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1:500 – 1:2500) and Immunoprecipitation. HeLa cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the Caper of human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.