Target of this antibody:
Alternative names of antibody target:
Heat shock 90 kDa protein 1A/1B, Heat shock 90 kDa protein 1/2, HSP90-1/HSP90-2, HSPA1A, HSPA1, HSPA1B
Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. A major function of HSP90 and other HSPs is to act as molecular chaperones. HSP90 forms a complex with glucocorticoid receptor (GR), rendering the non-ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP56 or cyclophilin-40, forming an aporeceptor complex. HSP90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an aporeceptor complex with estrogen and androgen receptors.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat.
E. coli-expressed recombinant human HSP90
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03 % ProClin®
This antibody has been succesfully tested for use in following methods:
Western blot, immunoprecipitation, immunohistochemistry.
The antibody can be used in Western blotting (0.5-4 µg/ml). Based on researchers feed back, the antibody also works well in immunoprecipitation (10-20 µg/ml) and immunohistochemistry (frozen sections, 10-20 µg/ml). However, the optimal concentrations should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.